Represented in the collection are optical microscopes from all over the world, from the end of the 18th century to the 1960s, including a stereo-microscope, polarizing microscopes, magnifying preparation glasses, projection microscopes, photo labs for microscopes, special condensers, measuring oculars, lighting lamps and various other accessories. The collection activity is primarily focused on the Czechoslovak products from the companies: Serb & Stys – Prague, Meopta and ER-HA Kroměříž.
However, it also includes foreign products, which emerged and were used during the existence of Czechoslovakia. The collection is being continuously added to and extended through systematic collection i.e. through donations from schools and institutions and purchases from physical and legal entities. A special group in this field are electron microscopes. This collection is unique in the Czech Republic as well as other European museums. It contains two basic types of this equipment: Tesla BS 242, BS 413, BS 500 and BS613 radiographic microscopes and Tesla BS 300, BS 301 and BS 350th scanning microscopes.